Riboflavin is a marker of vitamin B2 status (2).
Patient/subject: Prandial status may have some effect on concentrations of riboflavin, but has no effect on FMN.
Matrix: Riboflavin and FMN are most stable in EDTA plasma.
Volume: Minimum volume is 60 µL, but 200 µL is optimal and allows reanalysis.
Preparation and stability: The plasma fraction must be separated from the blood cells, preferentially within 30 minutes. Samples should be put on ice, and protected from light. Riboflavin is light sensitive. In serum, citrate plasma or heparin plasma, FMN is converted to riboflavin. Measurement of the sum of riboflavin plus FMN is an option in samples that have not been adequately treated.
Frozen, on dry ice. (for general instruction on transportation, click here.)
Reported values (riboflavin/FMN): 5-100/3-30 nmol/L.
Intraclass correlation coefficient (ICC) (riboflavin/FMN): 0.79 /0.69.
1. Midttun, O., Hustad, S., and Ueland, P.M. (2009). Quantitative profiling of biomarkers related to B-vitamin status, tryptophan metabolism and inflammation in human plasma by liquid chromatography/tandem mass spectrometry. Rapid Commun Mass Sp 23, 1371-79.
2. Hustad, S., McKinley, M.C., McNulty, H., Schneede, J., Strain, J.J., Scott, J.M., and Ueland, P.M. (2002). Riboflavin, flavin mononucleotide, and flavin adenine dinucleotide in human plasma and erythrocytes at baseline and after low-dose riboflavin supplementation. Clin Chem 48, 1571-77.
This website uses cookies. By continuing to use this site, you accept our use of cookies. Learn more
Â
Amino acids: Alanine, Arginine, Asparagine, Aspartic acid, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Kynurenine, Leucine, Lysine, Methionine, Ornithine, Phenylalanine, Proline, Sarcosine, Serine, Threonine, Total cysteine, Tryptophan, Tyrosine, Valine
Amino acids and catabolites: 2-Aminoadipic acid, 2-Hydroxybutyrate, 3-Hydroxysiobutyrate, α-Hydroxyglutaric acid, β-Alanine, β-Aminoisobutyrate, β-Hydroxy B-methylbutyric acid, Phenylacetylglutamine
Acylcarnities: BB, C0, C2, C3, C3, DC, C4, C4-OH , C4-DC , iC5 , C5-DC, C5:1 , C6 , C8, C10, C12, C14 , C14-OH, C16, C16-OH, C18 , C18-OH, C18:1, C18:2
TCA metabolites: α-Ketoglutarate, Citrate, Fumarate, Isocitrate, Lactate, Malate, Pyruvate, Ketone bodies, 3-Hydroxybutyrate, Acetoacetate
AGEs: Carboxyethyllysine, Carboxymethyllysine
Â
Kynurenines: Kynurenine, Kynurenic acid, Quinaldic acid, Anthranilic acid, 3-Hydroxykynurenine, Xanthurenic acid, 3-Hydroxyanthranilic acid, Picolinic acid, Quinolinic acid, Nicotinic acid, Nicotinamide, N1-methylnicotinamide
Neopterin
Proteins: C-Reactive protein, Calprotectin, Serum Amyloid A
Ratio-derived: Kynurenine/tryptophan ratio, PAr index (PLP, PL, PA)
Â
SCFAs: Acetate, Butyrate, Formate, Isobutyrate, Isovalerate, Propionate, Valerate, α-Methylbutyrate
Indoles: 3-Indoxyl sulfate, Imidazole propionate, Indole-3-acetamide, Indole-3-acetate, Indole-3-aldehyde, Indole-3-lactate, Indole-3-propionate
Choline metabolites: Choline ,Betaine, DMG, TMAO
Amino acid derived: Phenylacetylglutamine
Â
B-vitamins, functional markers, and methyl donors: 4-Pyridoxic acid, Betaine, Choline, Cobalamin, Flavin mononucleotide, Folate, Methylmalonic acid, N1-methylnicotinamide, Nicotinamide, Nicotinic acid, Pyridoxal, Pyridoxal 5-phosphate, Pyridoxine, Riboflavin, Thiamine, Thiamine monophosphate, Total homocysteine
Fat-soluble vitamins: 25-hydroxy vitamin D2, 25-hydroxy vitamin D3, α-Tocopherol (Vit. E), All-trans retinol (Vit. A), Phylloquinone (Vit. K1), y-Tocopherol (Vit. E)
Essential amino acids: Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Threonine, Tryptophan, Valine
Meat and fish intake: 1-Methylhistidine, 3-Methylhistidine, B-Alanine, Creatine, Creatinine, TMAO
Tobacco use and coffee intake: Cotinine, Trans-3-hydroxycotinine, Trigonelline
Please fill out the fields below (*required)
We will reply to you within 48hrs.
Thank you for reaching out to Bevital!
Please fill out the fields below (*required).
We will reply to you soon for project discussion.
Thank you for reaching out to Bevital!
Please fill out the fields below (*required).
We will reply to you within 48hrs.
Thank you for reaching out to Bevital!
Please fill out the fields below (*required)
We will reply to you within 48hrs.
Thank you for reaching out to Bevital!