Targeted Metabolomics Services


Updated 21/12/2022

What is dimethylglycine?

Dimethylglycine is formed from betaine during the betaine-homocysteine methyltransferase reaction. It is further degraded to monomethylglycine (sarcosine). Plasma dimethylglycine is associated with risk of acute myocardial infarction in patients with stable angina pectoris (2, 3).
Method: LC-MS/MS.


Assessment of choline and one-carbon status. Assessment of cardiovascular risk.

Specimen, collection and processing

Matrix: EDTA plasma is preferred if sarcosine is not measured simultaneously.
Volume: Minimum volume is 50 µL, but 200 µL is optimal and allows reanalysis.
Preparation and stability: The blood sample must be centrifuged and the plasma/serum fraction put on ice, and frozen.


Frozen, on dry ice. (for general instruction on transportation, click here)

Reported values, interpretation

Reported values: 1.5-5 µmol/L
Intraclass correlation coefficient (ICC): 0.64.


1. Midttun, O., Kvalheim, G., and Ueland, P.M. (2013). High-throughput, low-volume, multianalyte quantification of plasma metabolites related to one-carbon metabolism using HPLC-MS/MS. Anal Bioanal Chem 405, 2009-017.
2. Svingen, G.F., Ueland, P.M., Pedersen, E.K., Schartum-Hansen, H., Seifert, R., Ebbing, M., Løland, K.H., Tell, G.S., and Nygård, O. (2013). Plasma Dimethylglycine and Risk of Incident Acute Myocardial Infarction in Patients With Stable Angina Pectoris. Arterioscler Thromb Vasc Biol 33, 2041-48.
3. Svingen, G.F., Schartum-Hansen, H., Ueland, P.M., Pedersen, E.R., Seifert, R., Ebbing, M., Bønaa, K.H., Mellgren, G., Nilsen, D.W., et al. (2015). Elevated plasma dimethylglycine is a risk marker of mortality in patients with coronary heart disease. Eur J Prev Cardiol 22, 743-752

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