Patient/subject: Prandial status affects the concentration, which increases 10-15 % after a meal. The subjects should be fasting.
Matrix: EDTA plasma and serum.
Volume: Minimum volume is 50 µL, but 200 µL is optimal and allows reanalysis.
Preparation and stability: Betaine is fairly stable. The blood sample should be centrifuged and the plasma/serum fraction put on ice, and frozen.
Reported values: 15-51 µmol/L. The concentration is 20 % higher in men than in women.
Intraclass correlation coefficient (ICC) : 0.65.
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