Patient/subject: Prandial status affects the concentration, which increases 10-15 % after a meal. The subjects should be fasting.
Matrix: EDTA plasma and serum.
Volume: Minimum volume is 35 µL, but 200 µL is optimal and allows reanalysis.
Preparation and stability: Betaine is fairly stable. The blood sample should be centrifuged and the plasma/serum fraction put on ice, and frozen.
Reported values: 15-60 µmol/L. The concentration is 20 % higher in men than in women.
Intraclass correlation coefficient (ICC) : 0.65.
1. Midttun, O., Kvalheim, G., and Ueland, P.M. (2013). High-throughput, low-volume, multianalyte quantification of plasma metabolites related to one-carbon metabolism using HPLC-MS/MS. Anal Bioanal Chem 405, 2009-017.
2. Fernàndez-Roig, S., Cavallé-Busquets, P., Fernandez-Ballart, J.D., Ballesteros, M., Berrocal-Zaragoza, M.I., Salat-Batlle, J., Ueland, P.M., and Murphy, M.M. (2013). Low folate status enhances pregnancy changes in plasma betaine and dimethylglycine concentrations and the association between betaine and homocysteine. Am J Clin Nutr 97, 1252-59.
3. Holm, P.I., Ueland, P.M., Vollset, S.E., Midttun, O., Blom, H.J., Keijzer, M.B.A.J., and denHeijer, M. (2005). Betaine and folate status as cooperative determinants of plasma homocysteine in humans. Arterioscler Thromb Vasc Biol 25, 379-385.