Methylmalonic acid

Updated 22/11/2023

Indication(s)

Assessment of cobalamin status.
Method: GC-MS/MS

Specimen, collection and processing

Patient/subject: No special precaution.
Matrix: Serum or plasma.
Volume: Minimum volume is 50 µL, but 200 µL is optimal and allows reanalysis.
Preparation and stability: No special precaution. MMA is stable.

Transportation

Frozen, on dry ice. (for general instruction on transportation, click here.)

Reported values, interpretation

Reported values: < 0.26 µmol/L.
Intraclass correlation coefficient (ICC): 0.81.

Literature

1. Midttun, Ø., McCann, A., Aarseth, O., Krokeide, M., Kvalheim, G., Meyer, K., and Ueland, P.M. (2016). Combined measurement of 6 fat-soluble vitamins and 26 water-soluble functional vitamin markers and amino acids in 50 μL of serum or plasma by high-throughput mass spectrometry. Anal Chem 88, 10427-436.