BEVITAL AS

Targeted Metabolomics Services

Serum folate as pABG equivalents

pABG
Updated 21/12/2022

What is measured?

Synonyms: s-Folate.
Serum folate as pABG equivalents, after oxidation and mild acid hydrolysis.
Method(s): LC-MS/MS (1).

Methodological concern

The method involves quantitative conversion of all folate species and their polyglutamates into para-aminobenzoylglutamate (pABG) by oxidation and mild acid hydrolysis (2, 3). In fresh and adequately handled serum/plasma samples there is a strong correlation between serum folate measured as pABG equivalents and microbiologically active folate (2).

Indication(s)

Assessment of folate status, based on folate measured in serum/plasma samples exposed to elevated temperature or stored for decades (3).

Specimen, collection and processing

Patient/subject: Serum folate increases after at folate rich meal, and the concentration reflects (in contrast to erythrocyte folate) short-term folate statu.
Matrix: Serum or plasma.
Volume: Minimum volume is 60 µL, but 200 µL is optimal and allows reanalysis.

Transportation

Frozen, on dry ice. (for general instruction on transportation, click here.)

Reported values

Reported values: >7.5 nmol/L.
Intraclass correlation coefficients (ICC): 0.56.

Literature

1. Hannisdal, R., Ueland, P.M., and Svardal, A. (2009). Liquid chromatography-tandem mass spectrometry analysis of folate and folate catabolites in human serum. Clin Chem 55, 1147-154
2. Hannisdal, R., Svardal, A., and Ueland, P.M. (2008). Measurement of folate in fresh and archival serum samples as p-aminobenzoylglutamate equivalents. Clin Chem 54, 665-672.
3. Hannisdal, R., Gislefoss, R.E., Grimsrud, T.K., Hustad, S., Morkrid, L., and Ueland, P.M. (2010). Analytical recovery of folate and its degradation products in human serum stored at -25 degrees C for up to 29 years. J Nutr 140, 522-26.

This website uses cookies. By continuing to use this site, you accept our use of cookies.  Learn more

Scroll to Top

Join our Mailing list!

Get updates of our latest articles, events and method developments.