CRP belongs to the protein family of pentraxins, which is characterized by calcium dependent ligand-binding of five monomers of each 23kDa forming a radial symmetric ring. CRP is an acute phase reactant that responds as a sensitive, but non-specific marker for systemic inflammation. Elevated CRP (>10 ug/ml) have been related to higher risks of cardiovascular events, metabolic syndrome and cancer (2). Therefore, hsCRP has become an important biomarker and confounder in clinical and epidemiological studies.
Assessment of inflammation.
Matrix: Plasma and serum.
Volume: Minimum volume is 30 µL, but 100 µL is optimal and allows reanalysis.
Preparation: The blood sample must be centrifuged and the plasma/serum fraction put on ice, and frozen.
CRP is stable for 3 years at -20 °C, for 2 month at 4-8 °C.
Reported values: 0-10 µg/ml.
Intraclass correlation coefficient (ICC): 0.5-0.7.