Choline

choline

What is measured?

Free choline (as opposed to choline esters).
Method(s): LC-MS/MS (1).

Indication(s)

Assessment of one-carbon status. Choline deficiency.

Specimen, collection and processing

Patient/subject: Prandial status affects the concentration, which increases 10-15 % after a meal. The subjects should be fasting.
Matrix: EDTA plasma. The concentration increases in a time- and temperature-dependent manner in serum, due to formation from choline esters, the concentrations of which are 100 fold higher. This conversion is inhibited by EDTA.
Volume: Minimum volume is 50 µL, but 200 µL is optimal and allows reanalysis.
Preparation and stability: The blood sample must be immediately centrifuged and the plasma/serum fraction put on ice, and frozen. This is particularly important for serum samples.

Transportation

Frozen, on dry ice. (for general instruction on transportation, click here)

Reported values, interpretation

Reported values: 5-12 µmol/L.
Intraclass correlation coefficient (ICC) : 0.36.

Literature

1. Midttun, O., Kvalheim, G., and Ueland, P.M. (2013). High-throughput, low-volume, multianalyte quantification of plasma metabolites related to one-carbon metabolism using HPLC-MS/MS. Anal Bioanal Chem 405, 2009-017.

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