BEVITAL AS

Targeted Metabolomics Services

Calprotectin

Calprotectin-4GGF
Wikimedia Commons
Updated 21/12/2022

What is measured?

S100A8, S100A9, S100A9dm and S100A9tr. S100At is the total concentration of detected calprotectin isoforms.
Synonym: S100A8/9 or MRP8/14.
Method(s): MALDI-TOF MS (1).

What is calprotectin?

Calprotectin belongs to the S100 family of Ca-binding proteins and represents different hetero-complexes of S100A8, S100A9 and an isoform of truncated S100A9. The definition of the calprotectin hetero-complex is not consistent in the literature. The hetero-dimer has a molecular mass of about 24kDa. Binding of calcium and zinc determines the conformation, stability and function of the complexes. Calprotectin is highly expressed in neutrophils and other cells, and is classified as Damage Associated Molecular Pattern (DAMP) and interacting with TLR4 and possibly RAGE. While faecal calprotectin is widely used to diagnose inflammatory bowel diseases, quantification of calprotectin in serum or EDTA-plasma has recently become increasingly important for investigation of various inflammatory diseases as well as different types of cancers (2,3).

Indication(s)

Assessment of inflammation.

Specimen, collection and processing

Matrix: Serum, but preferably EDTA-plasma (2). See also stability curves for calprotectin.
Volume: Minimum volume is 30 µL, but 100 µL is optimal and allows reanalysis.
Preparation: The blood sample must be centrifuged and the plasma/serum fraction put on ice, and frozen.

Stability

Samples may be stored at 2-8 °C for up to 30 days and are stable at -20°C for at least 6 months.

Transportation

Frozen, on dry ice. (for general instruction on transportation, click here)

Reported values, interpretation

Reported values: 0-30 µg/ml.
Intraclass correlation coefficient (ICC): 0.3-0.5.

Literature

1. Gao, J., Meyer, K., Borucki, K., and Ueland, P.M. (2018). Multiplex immuno-MALDI-TOF MS for targeted quantification of protein biomarkers and their proteoforms related to inflammation and renal dysfunction. Anal Chem 90, 3366-373.
2. Pruenster, M., Vogl, T., Roth, J., and Sperandio, M. (2016). S100A8/A9: From basic science to clinical application. Pharmacol Ther 167, 120-131.
3. Nordal, H.H., Fagerhol, M.K., Halse, A.K., and Hammer, H.B. (2018). Calprotectin (S100A8/A9) should preferably be measured in EDTA-plasma; results from a longitudinal study of patients with rheumatoid arthritis. Scand J Clin Lab Invest 78, 102-08.

Scroll to Top
Cardiometabolic
Inflammation
Microbiome
Nutrition
Categories

Amino acids, amino acid catabolites, acylcarnitines, TCA metabolites, ketone bodies, AGEs



Kynurenines, ratio-derived metabolites, proteins





SCFAs, indoles, choline metabolites, amino acid derived





B-vitamins, functional markers, and methyl donors, fat-soluble vitamins, essential amino acids, meat & fish intake, tobacco use & coffee intake
Biomarkers (n)
65
18
20
41
View

Amino acids: Alanine, Arginine, Asparagine, Aspartic acid, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Kynurenine, Leucine, Lysine, Methionine, Ornithine, Phenylalanine, Proline, Sarcosine, Serine, Threonine, Total cysteine, Tryptophan, Tyrosine, Valine

Amino acid catabolites: 2-Aminoadipic acid, 2-Hydroxybutyrate, 3-Hydroxysiobutyrate, α-Hydroxyglutaric acid, β-Alanine, β-Aminoisobutyrate, β-Hydroxy B-methylbutyric acid, Phenylacetylglutamine

Acylcarnities: BB, C0, C2, C3, C3-DC, C4, C4-OH, C4-DC, iC5, C5-DC, C5:1, C6, C8, C10, C12, C14, C14-OH, C16, C16-OH, C18, C18-OH, C18:1, C18:2

TCA metabolites: α-Ketoglutarate, Citrate, Fumarate, Isocitrate, Lactate, Malate, Pyruvate

Ketone bodies: Acetoacetate, 3-Hydroxybutyrate

AGEs: Carboxyethyllysine, Carboxymethyllysine

View

Kynurenines: 3-hydroxykynurenine, 3-hydroxyanthranilic acid, Anthranilic acid, Kynurenine, Kynurenic acid, Nicotinic acid, Nicotinamide, N1-methylnicotinamide, Picolinic acid, Quinaldic acid, Quinolinic acid, Xanthurenic acid

Neopterin

Proteins: C-Reactive protein, Calprotectin, Serum Amyloid A

Ratio-derived: Kynurenine/tryptophan ratio, PAr index (PLP, PL, PA)

View

SCFAs: Acetate, Butyrate, Formate, Isobutyrate, Isovalerate, Propionate, Valerate, α-Methylbutyrate

Indoles: 3-Indoxyl sulfate, Imidazole propionate, Indole-3-acetamide, Indole-3-acetate, Indole-3-aldehyde, Indole-3-lactate, Indole-3-propionate

Choline metabolites: Choline, Betaine, DMG, TMAO

Amino acid derived: Phenylacetylglutamine

View

B-vitamins, functional markers, and methyl donors: 4-Pyridoxic acid, Betaine, Choline, Cobalamin, Flavin mononucleotide, Folate, Methylmalonic acid, N1-methylnicotinamide, Nicotinamide, Nicotinic acid, Pyridoxal, Pyridoxal 5-phosphate, Pyridoxine, Riboflavin, Thiamine, Thiamine monophosphate, Total homocysteine

Fat-soluble vitamins: 25-hydroxy vitamin D2, 25-hydroxy vitamin D3, α-Tocopherol (Vit. E), All-trans retinol (Vit. A), Phylloquinone (Vit. K1), y-Tocopherol (Vit. E)

Essential amino acids: Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Threonine, Tryptophan, Valine

Meat and fish intake: 1-Methylhistidine, 3-Methylhistidine, B-Alanine, Creatine, Creatinine, TMAO

Tobacco use and coffee intake: Cotinine, Trans-3-hydroxycotinine, Trigonelline

Volume (μl)
200
150
250
300
Analytical techniques
GC- and LC-MS/MS
LC- and MALDI-MS
GC- and LC-MS/MS
GC- and LC-MS/MS
Turnaround time (weeks)
1
1
1
1

Olink Proteomics

Please fill out the fields below (*required)
We will respond to you as soon as possible.
Thank you for reaching out to Bevital!

Customized analyses

Please fill out the fields below (*required).
We will reply to you soon for a detailed project discussion.
Thank you for reaching out to Bevital!

Mix-and-Match

Please fill out the fields below (*required).
We will respond to you as soon as possible.
Thank you for reaching out to Bevital!

Ready-to-Run

Please fill out the fields below (*required)
We will respond as soon as possible.
Thank you for reaching out to Bevital!

Thanks for joining!

Get updates about new method developments, publications and comming events.