Sarcosine

What is measured?

Synonym: Monomethylglycine.
Method(s): GC-MS/MS (1).

What is sarcosine?

Sarcosine is formed from dimethylglycine (dimethylglycine dehydrogenase) or by methylation of glycine (glycine-N-methyl transferase). It is degraded to glycine (sarcosine dehydrogenase).

Indication(s)

Assessment of choline and one-carbon status.

Specimen, collection and processing

Patient/subject: No special precaution.
Matrix: Sarcosine cannot be determined in samples collected into EDTA Vacutainer Tubes from some producer(s) (Becton, Dickinson), because of the presence of significant amounts of sarcosine in such tubes.
Volume: Minimum volume is 50 µL, but 200 µL is optimal and allows reanalysis.
Preparation and stability: The blood sample must be centrifuged and the plasma/serum fraction put on ice, and frozen.

Transportation

Frozen, on dry ice. (for general instruction on transportation, click here)

Reported values, interpretation

Reported values: 0.7-2.3 µmol/L
Intraclass correlation coefficient (ICC): 0.68.

Literature

1. Midttun, Ø., McCann, A., Aarseth, O., Krokeide, M., Kvalheim, G., Meyer, K., and Ueland, P.M. (2016). Combined measurement of 6 fat-soluble vitamins and 26 water-soluble functional vitamin markers and amino acids in 50 μL of serum or plasma by high-throughput mass spectrometry. Anal Chem 88, 10427-436.

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