Chemical structures and descriptions

β-Alanine

bAla

What is measured?

Synonyms: 3-Aminopropanoic acid, 3-aminopropionic acid.
Method(s): GC-MS/MS (1).

What is β-Alanine?

β-Alanine (bAla) is a naturally occurring beta-amino acid that is formed during degradation of carnosine and anserine, but also serves as a precursor for the synthesis of these histidine-containing dipeptides. This explains why beta-alanine may reflect the amount of carnosine/anserine in the body, and serves as a biomarker for the consumption of meat, especially red meat.
β-Alanine is used as a supplement. High levels, as encountered in some inborn errors of metabolism causing hyper-beta-alaninemia, may act as a neurotoxin and as a mitochondrial toxin. Circulating levels is inversely associated with dementia (2).

Indication(s)

To investigate the metabolomic signature of human diseases.

Specimen, collection and processing

Patient/subject: No special precaution.
Matrix: Serum or EDTA plasma.
Volume: Minimum volume is 50 µL, but 200 µL is optimal and allows reanalysis.
Preparation and stability: Probably stable.

Transportation

Frozen, on dry ice. (for general instruction on transportation, click here)

Reported values, interpretation

Reported values: 0.5-10 µmol/L
Intraclass correlation coefficient (ICC): na.

Literature

1. Midttun, Ø., McCann, A., Aarseth, O., Krokeide, M., Kvalheim, G., Meyer, K., and Ueland, P.M. (2016). Combined measurement of 6 fat-soluble vitamins and 26 water-soluble functional vitamin markers and amino acids in 50 μL of serum or plasma by high-throughput mass spectrometry. Anal Chem 88, 10427-436.
2. Hata, J., Ohara, T., Katakura, Y., Shimizu, K., Yamashita, S., Yoshida, D., Honda, T., Hirakawa, Y., Shibata, M., Sakata, S., Kitazono, T., Kuhara, S., & Ninomiya, T. (2019). Association Between Serum β-Alanine and Risk of Dementia. Am J Epidemiol, 188, 1637-164.

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Statistical power is the probability that a statistical test will correctly reject a false null hypothesis (H0​) when a specific alternative hypothesis (H1​) is true. H0​ is the null hypothesis, which states there is no effect or no difference. H1​ is the alternative hypothesis, which states there is a real effect or difference. Alpha (α) is the probability of a Type I error (a false positive), which is the risk of incorrectly rejecting the H0​ when it is actually true. You set this value before the experiment, commonly at 0.05. Beta (β) is the probability of a Type II error (a false negative), which is the risk of failing to reject the H0​ when it is actually false.

Power is calculated as 1−β. Increasing power means you are decreasing the probability of making a Type II error.

Several factors can be adjusted to increase the power of a statistical test:

  • Effect Size: This is the magnitude of the difference you are trying to detect. A larger effect size is easier to detect, thus increasing power. 

  • Sample Size: The number of observations in a study. A larger sample size provides more information about the population, reducing the margin of error and increasing the power to detect a true effect.

  • Variation: Refers to the spread or standard deviation of the data within the population. Less variation makes it easier to distinguish a real effect from random noise, thereby increasing power.

  • Alpha (): Increasing the alpha level (e.g., from 0.05 to 0.10) also increases power, but at the cost of a higher risk of a Type I error. This trade-off is often undesirable.

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561.

Vollset, S E; Nygârd, O; Refsum, H; Ueland, P M

Coffee and homocysteine Miscellaneous

2000, ISSN: 0002-9165.

Links | BibTeX

562.

Schneede, J; Refsum, H; Ueland, P M

Biological and environmental determinants of plasma homocysteine Journal Article

In: Semin Thromb Hemost, vol. 26, no. 3, pp. 263–279, 2000, ISSN: 0094-6176.

Abstract | Links | BibTeX

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